A freeze substitution fixation-based gold enlarging technique for EM studies of endocytosed Nanogold-labeled molecules.

@article{He2007AFS,
  title={A freeze substitution fixation-based gold enlarging technique for EM studies of endocytosed Nanogold-labeled molecules.},
  author={Wanzhong He and Christine Kivork and Suman Machinani and Mary Morphew and Anna M Gail and Devin B Tesar and Noreen E Tiangco and J Richard McIntosh and Pamela J. Bjorkman},
  journal={Journal of structural biology},
  year={2007},
  volume={160 1},
  pages={103-13}
}
We have developed methods to locate individual ligands that can be used for electron microscopy studies of dynamic events during endocytosis and subsequent intracellular trafficking. The methods are based on enlargement of 1.4 nm Nanogold attached to an endocytosed ligand. Nanogold, a small label that does not induce misdirection of ligand-receptor complexes, is ideal for labeling ligands endocytosed by live cells, but is too small to be routinely located in cells by electron microscopy… CONTINUE READING