A fractional programming approach to efficient DNA melting temperature calculation

@article{Leber2005AFP,
  title={A fractional programming approach to efficient DNA melting temperature calculation},
  author={Markus Leber and Lars Kaderali and Alexander Sch{\"o}nhuth and Rainer Schrader},
  journal={Bioinformatics},
  year={2005},
  volume={21 10},
  pages={
          2375-82
        }
}
MOTIVATION In a wide range of experimental techniques in biology, there is a need for an efficient method to calculate the melting temperature of pairings of two single DNA strands. Avoiding cross-hybridization when choosing primers for the polymerase chain reaction or selecting probes for large-scale DNA assays are examples where the exact determination of melting temperatures is important. Beyond being exact, the method has to be efficient, as these techniques often require the simultaneous… CONTINUE READING

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