A formula for thermal stability (Tm) prediction of PNA/DNA duplexes.

  title={A formula for thermal stability (Tm) prediction of PNA/DNA duplexes.},
  author={Ulla Giesen and W Kleider and Christoph Berding and A H Geiger and Henrik Orum and Peter E. Nielsen},
  journal={Nucleic acids research},
  volume={26 21},
An empirical formula for thermal stability (T m) prediction of PNA/DNA duplexes has been derived. The model is based on the T m as calculated for the corresponding DNA/DNA duplex employing a nearest neighbour approach, by including terms for the pyrimidine content and length of the PNA to take into account the increased thermostability of PNA/DNA hybrids and the asymmetry of the PNA-DNA heteroduplex. The predictive power of the T m prediction formula was challenged with an independent data set… CONTINUE READING

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Antisense Nucleic Acid Drug Dev., 7, 431–437

P. E. Nielsen, H. Ørum
Molecular Biology: Current Innovations and Future Trends, • 1995

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