A force-based protein biochip.

  title={A force-based protein biochip.},
  author={Kerstin Blank and T Mai and Ian Gilbert and Susi Schiffmann and Julia Rankl and Robert A. Zivin and Charles Tackney and Thomas Nicolaus and Klaus Spinnler and Filipp Oesterhelt and M Benoit and Hauke Clausen-Schaumann and Hermann Eduard Gaub},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={100 20},
A parallel assay for the quantification of single-molecule binding forces was developed based on differential unbinding force measurements where ligand-receptor interactions are compared with the unzipping forces of DNA hybrids. Using the DNA zippers as molecular force sensors, the efficient discrimination between specific and nonspecific interactions was demonstrated for small molecules binding to specific receptors, as well as for protein-protein interactions on protein arrays. Finally, an… CONTINUE READING


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