A food-grade system for inducible gene expression in Lactobacillus plantarum using an alanine racemase-encoding selection marker.

@article{Nguyen2011AFS,
  title={A food-grade system for inducible gene expression in Lactobacillus plantarum using an alanine racemase-encoding selection marker.},
  author={Tien-Thanh Nguyen and Geir Mathiesen and Lasse Fredriksen and Roman Kittl and Thu-Huong. Nguyen and Vincent G H Eijsink and Dietmar Haltrich and Clemens K. Peterbauer},
  journal={Journal of agricultural and food chemistry},
  year={2011},
  volume={59 10},
  pages={
          5617-24
        }
}
Food-grade gene expression systems for lactic acid bacteria are useful for applications in the food industry. We describe a new food-grade host/vector system for Lactobacillus plantarum based on pSIP expression vectors and the use of the homologous alanine racemase gene (alr) as selection marker. A new series of expression vectors were constructed by exchanging the erythromycin resistance gene (erm) in pSIP vectors by the L. plantarum WCFS1 alr gene. The vectors were applied for the… CONTINUE READING
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