A fluorometric method for estimating the calcium content of internal stores.

@article{Bergling1998AFM,
  title={A fluorometric method for estimating the calcium content of internal stores.},
  author={S Bergling and Ricardo E Dolmetsch and Richard S Lewis and Joel Keizer},
  journal={Cell calcium},
  year={1998},
  volume={23 4},
  pages={251-9}
}
The concentration of Ca2+ in intracellular stores is an important factor in many aspects of Ca2+ signaling, including the generation of Ca2+ spikes, oscillations and waves, control of mitochondrial respiration, and activation of store-operated Ca2+ channels. Here we describe a consistent method for estimating the content of stores, based on the release of stored Ca2+ by thapsigargin (TG) or ionomycin (IO). Once released from stores, Ca2+ elevates [Ca2+]i transiently before it is pumped across… CONTINUE READING

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