A fluorometric determination of N-methyl-4-phenylpyridinium ion, using high-performance liquid chromatography.

@article{Naoi1987AFD,
  title={A fluorometric determination of N-methyl-4-phenylpyridinium ion, using high-performance liquid chromatography.},
  author={Makato Naoi and Tsutomu Takahashi and Toshiharu Toshi Nagatsu},
  journal={Analytical biochemistry},
  year={1987},
  volume={162 2},
  pages={540-5}
}
A simple and sensitive assay procedure for the quantitation of N-methyl-4-phenylpyridinium ion (MPP+), a neurotoxin, was devised using its fluorescence and high-performance liquid chromatography (HPLC). The fluorescence intensity of MPP+ was several thousand times more than that of N-methyl-1,2,3,6-tetrahydropyridine (MPTP), a metabolic precursor of MPP+. This method was found to be sensitive enough to measure less than 10 pmol MPP+ without using HPLC and 10 fmol using HPLC. The oxidation of… CONTINUE READING