A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol.

@article{Stocks1986AFA,
  title={A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol.},
  author={Shannon K. Stocks and Andrew J. S. Jones and Charles Ramey and Donald Elliott Brooks},
  journal={Analytical biochemistry},
  year={1986},
  volume={154 1},
  pages={
          232-4
        }
}
An alternative primary amine assay utilizing 4-phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione (fluorescamine) is compared with the frequently applied trinitrobenzenesulfonic acid assay as a means of estimating protein primary amine modification with cyanuric chloride attachment of polyethylene glycol (PEG). The results of the two assays are compared for three different proteins and several advantages of the fluorescamine assay are emphasized. In particular, the fluorescamine assay was found to… CONTINUE READING
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