A fluorometric assay for measurement of mono-ADP-ribosyltransferase activity.

Abstract

Using 1,N6-etheno NAD, a fluorescent analog of NAD, we extended an existing assay for NAD glycohydrolase to the measurement of mono-ADP-ribosyltransferase (mADP-RT) activity using agmatine as acceptor for ADP-ribose. The reaction products were analyzed by reversed-phase chromatography. In the presence of agmatine two newly formed fluorescent products were… (More)

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Cite this paper

@article{Klebl1996AFA, title={A fluorometric assay for measurement of mono-ADP-ribosyltransferase activity.}, author={Bert M. Klebl and Drik Pette}, journal={Analytical biochemistry}, year={1996}, volume={239 2}, pages={145-52} }