A fluorogenic, mixed-disulfide reagent for thiol assay.

Abstract

The design and synthesis of the mixed disulfide (ortho-nitroaniline-N-ethyldithio) 2-[pyridyl-5-thioureido-N'-(5-fluorescein)] are described and the chemical and spectroscopic properties of this thiol-specific fluorogenic reagent are presented. The high reactivity and sensitivity of this reagent in thiol assay are demonstrated with low-molecular-weight… (More)

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Cite this paper

@article{Willis1986AFM, title={A fluorogenic, mixed-disulfide reagent for thiol assay.}, author={K. J. Willis and F W Teale}, journal={Analytical biochemistry}, year={1986}, volume={153 2}, pages={336-47} }