A fluorimetric method for measurement of erythrocyte transketolase activity.

Abstract

A method for measuring erythrocyte transketolase activity (ETKA) is described that is precise, economical of reagents and capable of high throughput with partial or full automation. The transketolase-dependent transformation of ribose-5-phosphate, yielding glyceraldehyde-3-phosphate, is linked via 'indicator enzymes' to glycerol production and NADH… (More)

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