A fluorimetric assay for hydrogen peroxide, suitable for NAD(P)H-dependent superoxide generating redox systems.

  title={A fluorimetric assay for hydrogen peroxide, suitable for NAD(P)H-dependent superoxide generating redox systems.},
  author={Robert M. Rapoport and Israel Hanukoglu and David J Sklan},
  journal={Analytical biochemistry},
  volume={218 2},
We report a simple and sensitive fluorimetric method for quantitative assay of the production rate of hydrogen peroxide, and indirectly of superoxide, during electron transfer reactions. The assay requires the inclusion of superoxide dismutase, catalase, and 6% methanol in the tested reaction system, to stochiometrically produce formaldehyde per molecule of H2O2 generated. The reaction is terminated by adding 2 vol of Nash reagent and heating at 60 degrees C for 10 min, to convert accumulated… 
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