A fluorescent reporter of caspase activity for live imaging.

  title={A fluorescent reporter of caspase activity for live imaging.},
  author={Pierre-Luc Bardet and Golnar Kolahgar and A. Mynett and Irene Miguel-Aliaga and James Briscoe and Pascal C. H. Meier and J. Oswald Vincent},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={105 37},
There is a growing interest in the mechanisms that control the apoptosis cascade during development and adult life. To investigate the regulatory events that trigger apoptosis in whole tissues, we have devised a genetically encoded caspase sensor that can be detected in live and fixed tissue by standard confocal microscopy. The sensor comprises two fluorophores, mRFP, monomeric red fluorescent protein (mRFP) and enhanced green fluorescent protein (eGFP), that are linked by an efficient and… CONTINUE READING


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