A fluorescent probe study of plasminogen activator inhibitor-1. Evidence for reactive center loop insertion and its role in the inhibitory mechanism.

@article{Shore1995AFP,
  title={A fluorescent probe study of plasminogen activator inhibitor-1. Evidence for reactive center loop insertion and its role in the inhibitory mechanism.},
  author={Joseph D. Shore and Duane E Day and A M Francis-Chmura and Ingrid M Verhamme and J O Kvassman and Daniel A Lawrence and David Ginsburg},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 10},
  pages={5395-8}
}
A mutant recombinant plasminogen activator inhibitor 1 (PAI-1) was created (Ser-338-->Cys) in which cysteine was placed at the P9 position of the reactive center loop. Labeling this mutant with N,N'-dimethyl-N-(acetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylene diamine (NBD) provided a molecule with a fluorescent probe at that position. The NBD-labeled mutant was almost as reactive as wild type but was considerably more stable. Complex formation with tissue or urokinase type plasminogen… CONTINUE READING

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