A fluorescence method to detect and quantitate sterol esterification by lecithin:cholesterol acyltransferase.

Abstract

We describe a simple but sensitive fluorescence method to accurately detect the esterification activity of lecithin:cholesterol acyltransferase (LCAT). The new assay protocol employs a convenient mix, incubate, and measure scheme. This is possible by using the fluorescent sterol dehydroergosterol (DHE) in place of cholesterol as the LCAT substrate. The… (More)
DOI: 10.1016/j.ab.2013.06.018

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Cite this paper

@article{Homan2013AFM, title={A fluorescence method to detect and quantitate sterol esterification by lecithin:cholesterol acyltransferase.}, author={Reynold Homan and Nadia Esmaeil and Laurel Mendelsohn and Gregory J. Kato}, journal={Analytical biochemistry}, year={2013}, volume={441 1}, pages={80-6} }