A fluorescence-based high-throughput screening assay for inhibitors of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H activity.

@article{Parniak2003AFH,
  title={A fluorescence-based high-throughput screening assay for inhibitors of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H activity.},
  author={Michael A. Parniak and Kyung-Lyum Min and Scott R. Budihas and Stuart F. J. Le Grice and John A Beutler},
  journal={Analytical biochemistry},
  year={2003},
  volume={322 1},
  pages={
          33-9
        }
}
A fluorescence resonance energy transfer assay readily applicable to 96-well and 384-well microplate formats with robotic operation was developed to enable high-throughput screening for inhibitors of human immunodeficiency virus-1 (HIV-1) reverse transcriptase (RT)-associated RNase H activity, an underexplored target for antiretroviral development. The assay substrate is an 18-nucleotide 3'-fluorescein-labeled RNA annealed to a complementary 18-nucleotide 5'-Dabcyl-modified DNA. The intact… CONTINUE READING
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