A fluorescence assay to determine the viable biomass of microcosm dental plaque biofilms.

@article{Filoche2007AFA,
  title={A fluorescence assay to determine the viable biomass of microcosm dental plaque biofilms.},
  author={Sara K Filoche and Megan J Coleman and Linny Angker and Chris H Sissons},
  journal={Journal of microbiological methods},
  year={2007},
  volume={69 3},
  pages={489-96}
}
Dental plaque bacteria form complex and robust cell aggregates which cannot be counted accurately using epifluorescence microscopy. This causes a significant problem for quantifying their viability. The aim of the investigation was to develop a fluorescence assay to quantify the viable biomass of dental plaque biofilms. Using an artificial mouth system, microcosm plaques were grown under a range of fluoride and mineralizing conditions, and were treated with the oral antiseptics chlorhexidine… CONTINUE READING