A flow cytometry-based immuno-titration assay for rapid and accurate titer determination of modified vaccinia Ankara virus vectors.

@article{Li2010AFC,
  title={A flow cytometry-based immuno-titration assay for rapid and accurate titer determination of modified vaccinia Ankara virus vectors.},
  author={Zengji Li and Loni Ling and Xiaohui Liu and Reiner Laus and Alain Delcayre},
  journal={Journal of virological methods},
  year={2010},
  volume={169 1},
  pages={
          87-94
        }
}
A flow cytometry-based immuno-titration titer assay was established to determine infectious unit (IU) and transducing unit (TU) of modified vaccinia Ankara (MVA) virus vectors. This titration method enumerates infected cells by measuring the expression of viral protein for IU and transgene protein for TU in individual cells after staining with fluorophore-conjugated antibodies. It presents many advantages over standard virus titration approaches, such as TCID(50) or plaque assay, for its… CONTINUE READING

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