A flow cytometric opsonophagocytic assay for measurement of functional antibodies elicited after vaccination with the 23-valent pneumococcal polysaccharide vaccine.

@article{Martinez1999AFC,
  title={A flow cytometric opsonophagocytic assay for measurement of functional antibodies elicited after vaccination with the 23-valent pneumococcal polysaccharide vaccine.},
  author={Joseph E Martinez and Sandra Romero-Steiner and Tamara Pilishvili and Suzanne Barnard and J Schinsky and David Goldblatt and George M. Carlone},
  journal={Clinical and diagnostic laboratory immunology},
  year={1999},
  volume={6 4},
  pages={581-6}
}
Opsonophagocytosis is the primary mechanism for clearance of pneumococci from the host, and the measurement of opsonophagocytic antibodies appears to correlate with vaccine-induced protection. We developed a semiautomated flow cytometric opsonophagocytosis assay using HL-60 granulocytes as effector cells and nonviable 5, 6-carboxyfluorescein, succinimidyl ester-labeled Streptococcus pneumoniae (serotypes 4, 6B, 9V, 14, 18C, 19F, and 23F) as bacterial targets. The flow cytometric… CONTINUE READING
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