A five-minute DNA extraction method for expedited detection of Phytophthora ramorum following prescreening using Phytophthora spp. lateral flow devices.

@article{Tomlinson2010AFD,
  title={A five-minute DNA extraction method for expedited detection of Phytophthora ramorum following prescreening using Phytophthora spp. lateral flow devices.},
  author={Jenny A. Tomlinson and Miles Dickinson and Ellie Hobden and Sally Robinson and Patricia M. Giltrap and Neil Boonham},
  journal={Journal of microbiological methods},
  year={2010},
  volume={81 2},
  pages={116-20}
}
In a direct comparison with established methods for Phytophthora ramorum detection (isolation followed by morphological identification, or conventional DNA extraction followed by TaqMan real-time PCR) a rapid, simplified detection method in which membranes of lateral flow devices (LFDs) are added directly to TaqMan real-time PCR reactions was used to test 202 plant samples collected by plant health inspectors in the field. P. ramorum prevalence within the 202 samples was approximately 40… CONTINUE READING