A fission yeast gene encoding a protein that preferentially associates with curved DNA.

@article{Yamada1994AFY,
  title={A fission yeast gene encoding a protein that preferentially associates with curved DNA.},
  author={Hisami Yamada and Hirotada Mori and H Momoi and Yoshiyuki Nakagawa and Chiharu Ueguchi and Takeshi Mizuno},
  journal={Yeast},
  year={1994},
  volume={10 7},
  pages={883-94}
}
We searched for fission yeast (Schizosaccharomyces pombe) proteins that preferentially bind to a synthetic curved DNA sequence, by means of a DNA-binding gel shift assay in the presence of an excess amount of a non-curved DNA sequence as a competitor. We identified such a protein in S. pombe. The protein, thus purified, has an apparent molecular weight of 42,000, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It was suggested that this protein (42 K-protein… CONTINUE READING