A fish is not a mouse: understanding differences in background genetics is critical for reproducibility

@article{Crim2020AFI,
  title={A fish is not a mouse: understanding differences in background genetics is critical for reproducibility},
  author={Marcus J Crim and Christian Lawrence},
  journal={Lab Animal},
  year={2020},
  volume={50},
  pages={19-25}
}
Poorly controlled background genetics in animal models contributes to the lack of reproducibility that is increasingly recognized in biomedical research. The laboratory zebrafish, Danio rerio , has been an important model organism for decades in many research areas, yet inbred strains and traditionally managed outbred stocks are not available for this species. Sometimes incorrectly referred to as ‘inbred strains’ or ‘strains’, zebrafish wild-type lines possess background genetics that are often… Expand
2 Citations
Molecular and culture-based assessment of the microbiome in a zebrafish (Danio rerio) housing system during set-up and equilibration
TLDR
The microbiome of commercial RAS used for research zebrafish reaches a relatively stable state by four weeks post-population and would be expected to be suitable for experimental use following that time-point. Expand
The Lymphatic System in Zebrafish Heart Development, Regeneration and Disease Modeling
TLDR
The findings in this review are discussed in the wider context of lymphatic development, evolution and the promise of this system to open new therapeutic avenues to treat myocardial infarction and other cardiopathologies. Expand

References

SHOWING 1-10 OF 71 REFERENCES
Genetic backgrounds, standard lines, and husbandry of zebrafish.
TLDR
This chapter outlines the current issues surrounding the construction, maintenance, and use of genetically defined lines of zebrafish, and the extensive use of forward genetic mutagenesis screens. Expand
Zebrafish breeding in the laboratory environment.
TLDR
A wild-type hybrid Sanger AB Tübingen line has been generated from sequenced homozygous founders, and a robust zebrafish line typically carries a large number of polymorphic variations, which may interfere with reproducibility of experiments. Expand
Behavioral research in zebrafish (Danio rerio): strain as source of variation
TLDR
It is shown that strain is a source of variation that should be taken into account to enhance replicability of data, and differences between AB and TL are highlighted, the focus of recent studies. Expand
Generation of Highly Homogeneous Strains of Zebrafish Through Full Sib-Pair Mating
TLDR
The attempted inbreed of two zebrafish wild-type strains, Tübingen and India, through full sib-pair mating suggests that extremely homogeneous zebra fish strains have been established, thereby creating a valuable resource for practical application. Expand
Population genetic diversity in zebrafish lines
TLDR
T5D is established as a model that is representative of diversity levels within laboratory zebrafish lines and demonstrated that experimental design and analysis can exert major effects when characterizing genetic diversity in heterogeneous populations. Expand
Genetic Monitoring of Laboratory Rodents
Publisher Summary This chapter reviews the problems associated with the maintenance of standardized strains of laboratory rodents and highlights the need to control their genetic quality and healthExpand
Assessment of polymorphism in zebrafish mapping strains.
TLDR
Levels of polymorphism between the inbred, C32 and SJD, strains and three other commonly used strains, the *AB, WIK, and Florida wild type (hereafter referred as EKK), are established, with CA-repeat markers as well as SSCP polymorphisms. Expand
Which Zebrafish Strains Are More Suitable to Perform Behavioral Studies? A Comprehensive Comparison by Phenomic Approach
TLDR
Each zebrafish strain tested in this experiment showed specifically different behaviors from each other, thus, strain-specific zebra Fish behavior should be considered when designing experiments using zebra fish behavior. Expand
The case for genetic monitoring of mice and rats used in biomedical research
TLDR
In this review, a case is made for the International Council for Laboratory Animal Science (ICLAS), which is interested in promoting and helping academic institutions develop a genetic monitoring program to bring a level of genetic quality assurance into the scientific interchange and use of mouse and rat genetically mutant models. Expand
A Defined Zebrafish Line for High-Throughput Genetics and Genomics: NHGRI-1
TLDR
NHGRI-1, a healthy and fecund strain derived from founder parents, is generated, and within this strain, the majority of the genome that matches the reference sequence is identified and documented and most of the variants are documented. Expand
...
1
2
3
4
5
...