A filter affinity transfer method for the analysis of rheumatoid factors.


Transfer of serum proteins separated by thin layer agarose electrophoresis onto nitrocellulose sheets precoated with purified human polyclonal IgG followed by revelation with enzyme-coupled anti-mu or anti-alpha antisera resulted in the specific detection of rheumatoid factors (RF) belonging to the IgM or IgA classes. Mono- or polyclonality of such RF can… (More)