A fast workflow for identification and quantification of proteomes.

  title={A fast workflow for identification and quantification of proteomes.},
  author={Chen Ding and Jing Jiang and Junying Wei and Wanlin Liu and Wei Zhang and Mingwei Liu and Tianyi Fu and Tianyuan Lu and Lei Song and Wantao Ying and Cheng Chang and Yangjun Zhang and Jie Ma and Lai Wei and Anna Malovannaya and Lijun Jia and Bei Zhen and Yi Wang and Fuchu He and Xiaohong Qian and Jun Qin},
  journal={Molecular & cellular proteomics : MCP},
  volume={12 8},
The current in-depth proteomics makes use of long chromatography gradient to get access to more peptides for protein identification, resulting in covering of as many as 8000 mammalian gene products in 3 days of mass spectrometer running time. Here we report a fast sequencing (Fast-seq) workflow of the use of dual reverse phase high performance liquid chromatography - mass spectrometry (HPLC-MS) with a short gradient to achieve the same proteome coverage in 0.5 day. We adapted this workflow to a… CONTINUE READING
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