A fast and selective two-photon phosphorescent probe for the imaging of nitric oxide in mitochondria.

@article{Chen2015AFA,
  title={A fast and selective two-photon phosphorescent probe for the imaging of nitric oxide in mitochondria.},
  author={Xiang Chen and Lingli Sun and Yu Chen and Xiaoling Cheng and Weijun Wu and Liang‐nian Ji and Hui Chao},
  journal={Biomaterials},
  year={2015},
  volume={58},
  pages={
          72-81
        }
}

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References

SHOWING 1-10 OF 65 REFERENCES
Visualization of nitric oxide in living cells by a copper-based fluorescent probe
TLDR
Using spectroscopic and mass spectrometric methods, it is established that the mechanism of the reaction responsible for the NO-induced fluorescence involves reduction of the complex to Cu(I) with release of the nitrosated ligand, which occurs irreversibly.
Quinoline-based two-photon fluorescent probe for nitric oxide in live cells and tissues.
A two-photon fluorescent probe (QNO) for nitric oxide is reported. The probe is designed with a photoinduced electron transfer (PeT) mechanism and shows 12-fold fluorescence enhancement toward NO.
Highly sensitive low-background fluorescent probes for imaging of nitric oxide in cells and tissues.
TLDR
The lipophilicity-based intracellular retention mechanism as a design strategy has great potential in the development of fluorescent probes for bioimaging and the high sensitivity and dark background make DANPBO-H and DAPBO-M competitive for NO imaging in cells and tissues.
Direct evidence of nitric oxide presence within mitochondria.
TLDR
These findings demonstrate for the first time that the subcellular distribution of NO production is consistent with the presence of a mitochondrial NOS, and provide a new tool to directly study the modulatory role of NO in mitochondrial respiration and membrane potential, in vivo.
Two-Photon Fluorescence Excitation Cross Sections of Biomolecular Probes from 690 to 960 nm.
TLDR
F fluorophore fluorescence intensities showed no significant departure from quadratic illumination power dependence, indicating genuine two-photon processes, and implications for two- photon laser-scanning microscopy are discussed.
Nitric oxide turn-on fluorescent probe based on deamination of aromatic primary monoamines.
TLDR
Cell imaging results demonstrated that FA-OMe could be used as a turn-on fluorescent sensor for the detection of endogenously generated NO and had high specificity for NO over other reactive oxygen/nitrogen species.
The rational design of a highly sensitive and selective fluorogenic probe for detecting nitric oxide.
TLDR
A rationally designed small-molecule fluorogenic probe for nitric oxide (NO) detection based on a new switching mechanism has been developed that displays a very high sensitivity to NO concentrations down to the low nM range and a veryhigh specificity to NO while being insensitive to other oxidative oxygen/nitrogen species that often interfere with the sensing of NO.
Development of a ruthenium(II) complex based luminescent probe for imaging nitric oxide production in living cells.
TLDR
The probe was successfully used for luminescence-imaging detection of the exogenous NO in mouse macrophage cells and endogenous NO in gardenia cells and demonstrated the efficacy and advantages of the new probe for NO detection in living cells.
...
...