A fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection

@inproceedings{Ferrari2014AFS,
  title={A fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection},
  author={Alessandro R Ferrari and Yasser Gaber and Marco W Fraaije},
  booktitle={Biotechnology for biofuels},
  year={2014}
}
BACKGROUND Most of the current colorimetric methods for detection of chitinase or cellulase activities on the insoluble natural polymers chitin and cellulose depend on a chemical redox reaction. The reaction involves the reducing ends of the hydrolytic products. The Schales' procedure and the 3,5-dinitrosalicylic acid (DNS) method are two examples that are commonly used. However, these methods lack sensitivity and present practical difficulties of usage in high-throughput screening assays as… CONTINUE READING
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