A facile procedure for purifying maize chloroplast RNA polymerase from whole cell homogenates.

@article{Kidd1980AFP,
  title={A facile procedure for purifying maize chloroplast RNA polymerase from whole cell homogenates.},
  author={George H. Kidd and L. Bogorad},
  journal={Biochimica et biophysica acta},
  year={1980},
  volume={609 1},
  pages={14-30}
}
Two DNA-dependent RNA polymerases have been purified from homogenates of maize leaves by a relatively rapid procedure involving Sepharose 4B and DEAE-cellulose chromatography followed by resolution of two RNA polymerasies on phosphocellulose. The RNA polymerase eluting from phosphocellulose at 0.11 M (NH4)2SO4 is inhibited strongly by low levels of alpha-amanitin and possesses catalytic properties and polypeptide subunits like those of maize nuclear RNA polymerase II. The RNA polymerase eluting… CONTINUE READING