A facile method for preparation of tailored scaffolds for DNA-origami.

Abstract

A convenient PCR cloning strategy allows one to prepare hundreds of picomoles of circular single-stranded DNA molecules, which are suitable as scaffolds for the assembly of DNA origami structures. The method is based on a combination of site-directed mutagenesis and site- and ligation-independent cloning protocols, with simultaneous insertion of a nicking… (More)
DOI: 10.1002/smll.201300701

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@article{Erkelenz2014AFM, title={A facile method for preparation of tailored scaffolds for DNA-origami.}, author={Michael Erkelenz and Dennis M Bauer and Rebecca Meyer and Christos Gatsogiannis and Stefan Raunser and Barbara Sacc{\`a} and Christof M Niemeyer}, journal={Small}, year={2014}, volume={10 1}, pages={73-7} }