A facile graphene oxide-based DNA polymerase assay.

@article{Xu2012AFG,
  title={A facile graphene oxide-based DNA polymerase assay.},
  author={Fengzhou Xu and Hui Shi and Xiaoxiao He and Kemin Wang and Xiaosheng Ye and Lv'an Yan and Shuyong Wei},
  journal={The Analyst},
  year={2012},
  volume={137 17},
  pages={3989-94}
}
The DNA polymerase assay is fundamental for related molecular biology investigations and drug screenings, however, the commonly used radioactive method is laborious and restricted. Herein, we report a novel, simple and cost-effective fluorometric DNA polymerase detection method by utilizing graphene oxide (GO) as a signal switch. In this strategy, in the absence of DNA polymerase, the fluorophore-labeled template ssDNA could be strongly adsorbed and almost entirely quenched by GO. However, as… CONTINUE READING
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