A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture

@article{Leivo2017ADA,
  title={A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture},
  author={Joni Leivo and Sanni Virjula and Sari Vanhatupa and Kimmo Kartasalo and Joose M. Kreutzer and Susanna Miettinen and Pasi Kallio},
  journal={Journal of The Royal Society Interface},
  year={2017},
  volume={14}
}
Polydimethylsiloxane (PDMS) is widely used in dynamic biological microfluidic applications. As a highly hydrophobic material, native PDMS does not support cell attachment and culture, especially in dynamic conditions. Previous covalent coating methods use glutaraldehyde (GA) which, however, is cytotoxic. This paper introduces a novel and simple method for binding collagen type I covalently on PDMS using ascorbic acid (AA) as a cross-linker instead of GA. We compare the novel method against… 
Surface Modification Techniques for Endothelial Cell Seeding in PDMS Microfluidic Devices
TLDR
This review collates different techniques with their advantages and disadvantages that have been used to improve PDMS hydrophilicity to facilitate endothelial cells seeding in PDMS devices.
Functionalization and Surface Modifications of Bioactive Glasses (BGs): Tailoring of the Biological Response Working on the Outermost Surface Layer
TLDR
An overview of the currently used approaches of surface functionalization and modifications of BGs are presented, along with discussing the biological outputs induced by these changes.
Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
TLDR
A transparent mechanical stimulation device capable of uniaxial stimulation, which is compatible with standard bioanalytical methods used in cellular mechanobiology, is presented and it is demonstrated that sarcomere structures of hiPSC-CMs organize and orient perpendicular to uniaXial strain axis and thus express more maturated nature of cardiomyocytes.
Marangoni flows drive the alignment of fibrillar cell-laden hydrogels
Cell-laden hydrogels can be engineered using evaporating droplets of collagen. When a sessile droplet containing a solute in a volatile solvent evaporates, flow in the droplet can transport and
SURFACE FUNCTIONALIZATION OF A SILICON NANOWIRE FIELD-EFFECT TRANSISTOR (SINW-FET) FOR miRNA DETECTION
TLDR
To enable miRNA SiNW-FET reusability, the thesis proposes thermal-, chemical-, and enzymes-based methods to detach the miRNA molecules from the DNA probes.
Homing Choices of Breast Cancer Cells Revealed by Tissue Specific Invasion and Extravasation Lab-on-a-chip Platforms
TLDR
Overall, this study presents IC-chip and EX-chip that can determine tissue-specific invasion and extravasation potentials of cancer cells providing the groundwork for novel diagnostic tools to predict metastasis risk.
On‐chip determination of tissue‐specific metastatic potential of breast cancer cells
TLDR
Results show that IC-chip and EX-chip, simulating tissue specific microenvironments, can distinguish different in vivo metastatic phenotypes, in vitro.

References

SHOWING 1-10 OF 55 REFERENCES
Chemical and physical modifications to poly(dimethylsiloxane) surfaces affect adhesion of Caco-2 cells.
TLDR
This study provides a foundation for further miniaturization, utilizing soft lithography techniques, of Caco-2 cell-based system for high-throughput screening of drug intestinal absorption during lead optimization in drug discovery.
Simple poly(dimethylsiloxane) surface modification to control cell adhesion
Poly(dimethylsiloxane) (PDMS) has a long history of exploitation in a variety of biological and medical applications. Particularly in the past decade, PDMS has attracted interest as a material for
Surface chemical modification of poly(dimethylsiloxane) for the enhanced adhesion and proliferation of mesenchymal stem cells.
TLDR
The covalent surface chemical modification of PDMS with APTES + GA + protein could offer a more biocompatible platform for the enhanced adhesion and proliferation of MSCs.
Spatial control of cell adhesion and patterning through mussel-inspired surface modification by polydopamine.
TLDR
The spatial control and patterning of mammalian cells were achieved by using the universal adhesive property of mussel-inspired polydopamine (PDA) and it was found that various cells predominantly adhered to PDA-modified regions, maintaining their normal morphologies.
Stable immobilization of rat hepatocytes as hemispheroids onto collagen‐conjugated poly‐dimethylsiloxane (PDMS) surfaces: Importance of direct oxygenation through PDMS for both formation and function
TLDR
It is demonstrated that a combination of collagen covalently immobilized PDMS and an adequate oxygen supply enables the establishment of a stable, attached spheroid (hemispheroid) culture of rat hepatocytes.
...
...