A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture

  title={A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture},
  author={Joni Leivo and Sanni Virjula and Sari Vanhatupa and Kimmo Kartasalo and Joose M. Kreutzer and Susanna Miettinen and Pasi Kallio},
  journal={Journal of The Royal Society Interface},
Polydimethylsiloxane (PDMS) is widely used in dynamic biological microfluidic applications. As a highly hydrophobic material, native PDMS does not support cell attachment and culture, especially in dynamic conditions. Previous covalent coating methods use glutaraldehyde (GA) which, however, is cytotoxic. This paper introduces a novel and simple method for binding collagen type I covalently on PDMS using ascorbic acid (AA) as a cross-linker instead of GA. We compare the novel method against… 
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