A duplex PCR assay for simultaneous detection and differentiation of Capripoxvirus and Orf virus.

@article{Zheng2007ADP,
  title={A duplex PCR assay for simultaneous detection and differentiation of Capripoxvirus and Orf virus.},
  author={Min Zheng and Qi Liu and N Zhou F Tuffner Z Huang S Jin and Jiangang Guo and Xia Huang and Huaming Li and Wei Zhu and Yi Xiong},
  journal={Molecular and cellular probes},
  year={2007},
  volume={21 4},
  pages={276-81}
}
A duplex polymerase chain reaction (PCR) was developed and optimized for simultaneous detection and differentiation of Capripoxvirus (CPV) (including goat pox virus and sheep pox virus) and orf virus (ORFV). Two sets of specific oligonucleotide primers were designed and used for CPV and ORFV, respectively. The duplex PCR DNA products, which consisted of fragments of 413 bp for A29L gene of CPV, and 708 bp for P55 gene of ORFV, were visualized by gel electrophoresis. The developed assay was… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 25 extracted citations

Similar Papers

Loading similar papers…