A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels.

@article{Liu2013ADF,
  title={A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels.},
  author={Nianwu Liu and Shijia Wu and Ye Yu and Xiaoyuan Ma and Yu Xia and Xiujuan Chen and Yukun Huang and Zhouping Wang},
  journal={Analytica chimica acta},
  year={2013},
  volume={804},
  pages={
          151-8
        }
}
A sensitive, specific method for the collection and detection of pathogenic bacteria was demonstrated using quantum dots (QDs) as a fluorescence marker coupled with aptamers as the molecular recognition element by flow cytometry. The aptamer sequences were selected using a bacterium-based SELEX strategy in our laboratory for Vibrio parahaemolyticus and Salmonella typhimurium that, when applied in this method, allows for the specific recognition of the bacteria from complex mixtures including… CONTINUE READING
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