A double-stranded molecular probe for homogeneous nucleic acid analysis.

@article{Meserve2008ADM,
  title={A double-stranded molecular probe for homogeneous nucleic acid analysis.},
  author={Daniel Meserve and Zhaohui Wang and Donna D. Zhang and Pak Kin Wong},
  journal={The Analyst},
  year={2008},
  volume={133 8},
  pages={1013-9}
}
This paper reports the design and optimization of a double-stranded molecular probe for homogeneous detection of specific nucleotide sequences. The probes are labeled with either a fluorophore or a quencher such that the probe hybridization brings the two labels into close proximity, and this diminishes the fluorescence signal in the absence of a target. In… CONTINUE READING