A double lead stain method for enhancing contrast of ultrathin sections in electron microscopy: a modified multiple staining technique.

A modification of the conventional method for the staining of ultrathin sections resulted in an increase in contrast of ultrastructural detail in tissues. Tissues embedded in Spurr's low viscosity embedding medium were stained with freshly centrifuged Reynolds' lead citrate for 1-5 min, rinsed in double distilled water and dried prior to staining with a… CONTINUE READING