A double-enzyme-coupled assay for high-throughput screening of succinic acid-producing strains.

Abstract

AIMS To develop a microplate-based high-throughput assay for the detection of succinic acid, which is widely used in many fields of industry. The assay can also be applied to screen high succinic acid-producing strains. METHODS AND RESULTS Here, we developed a double-enzyme-based succinic acid detection assay using fumarate reductase and peroxidase (named… (More)
DOI: 10.1111/jam.12175

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