[A double-antigen sandwich ELISA for detecting Penicillium marneffei Mp1p-specific antibody].

Abstract

OBJECTIVE To establish an immunological method for detecting antibodies of Penicillium marneffei. METHODS The recombinant Mp1p protein of Penicillium marneffei was expressed in Pichia pastoris and labeled with HRP (Mp1p-HRP) with a modified sodium periodate method. A double-antigen sandwich enzyme-linked immunosorbant assay (ELISA) was established by determining the optimal coating concentration of Mp1p protein and the concentration of the detecting protein Mp1p-HRP. The sensitivity and specificity of the assay was evaluated by detecting Mp1p antibodies in 100 serum samples from healthy donors, 15 samples from culture-confirmed penicilliosis patients, and 21 samples from patients with culture-confirmed other fungal infections. RESULTS A double-antigen sandwich ELISA was successfully established for detecting Mp1p-specific antibody. The specificity of the assay was 100% (121/121) for detecting Mp1p-specific antibody in the sera from healthy donors and patients with other fungal infection. The detection results of the 15 serum samples from patients with culture-confirmed penicilliosis showed positivity for Mp1p antibody in 2 samples and Mp1p antigen positivity in 12 samples; combining the detection results of Mp1p antigen and antibody obviously increased the diagnostic sensitivity to 93.3% (14/15). CONCLUSION The double-antigen sandwich ELISA shows a high specificity in detecting Mp1p-specific antibody, and simultaneous detection of Mp1p antigen and antibody can increase the diagnostic sensitivity for penicilliosis.

Cite this paper

@article{Wang2013ADS, title={[A double-antigen sandwich ELISA for detecting Penicillium marneffei Mp1p-specific antibody].}, author={Yanfang Wang and Lei Zeng and Xuedong Chen and Wei Hao and Mei Yang and Jianpiao Cai and Yadi Wang and Guoyong Yuan and Xiao-yan Che}, journal={Nan fang yi ke da xue xue bao = Journal of Southern Medical University}, year={2013}, volume={33 3}, pages={439-43} }