A double antibody sandwich micro-ELISA kit for the rapid diagnosis of snake bite.

@article{Dhaliwal1983ADA,
  title={A double antibody sandwich micro-ELISA kit for the rapid diagnosis of snake bite.},
  author={Jasbir Singh Dhaliwal and Toon Wei Lim and K D Sukumaran},
  journal={The Southeast Asian journal of tropical medicine and public health},
  year={1983},
  volume={14 3},
  pages={367-73}
}
A micro ELISA assay was established to diagnose systemic poisoning for the rapid administration of specific antivenom. Rabbit anti venom IgG was bound to the solid phase to enable detection of venom from both the Malayan Pit Viper (Agkistrodon rhodostoma) and the Common Cobra (Naja naja). This assay is read visually and takes 35 to 45 minutes to perform. It can detect 15.6 ng/ml of viper venom in 75 minutes and 7.8 ng/ml of cobra venom in 55 minutes. Tests on sera from snake bite patients… CONTINUE READING

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