A dominant negative Raf-1 mutant prevents v-Src-induced transformation.


A vector expressing a dominant negative mutant of Raf-1 was stably introduced into BALB/c 3T3 cells expressing a temperature-sensitive derivative of v-Src. High levels of the Raf-mutant were detected in these cells. Expression of the Raf-1 mutant blocked v-Src-induced transformation, as determined by reversion to a flat non-transformed morphology and the inability to form colonies in soft agar. Cells transfected with the parental vector lacking the mutant Raf-1 could be transformed by v-Src. These data suggest that intracellular signals activated by v-Src that are mediated by Raf-1 are required for transformation by v-Src.


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@article{Qureshi1993ADN, title={A dominant negative Raf-1 mutant prevents v-Src-induced transformation.}, author={Sohail Asif Qureshi and Cecil K Joseph and Mary K. Hendrickson and Junmin Song and Ruchika Gupta and J T Bruder and Ulf R{\"{u}diger Rapp and David A. Foster}, journal={Biochemical and biophysical research communications}, year={1993}, volume={192 2}, pages={969-75} }