A distinct tethering step is vital for vacuole membrane fusion

@inproceedings{Zick2014ADT,
  title={A distinct tethering step is vital for vacuole membrane fusion},
  author={Michael Zick and William Wickner and Axel T Brunger},
  booktitle={eLife},
  year={2014}
}
Past experiments with reconstituted proteoliposomes, employing assays that infer membrane fusion from fluorescent lipid dequenching, have suggested that vacuolar SNAREs alone suffice to catalyze membrane fusion in vitro. While we could replicate these results, we detected very little fusion with the more rigorous assay of lumenal compartment mixing. Exploring the discrepancies between lipid-dequenching and content-mixing assays, we surprisingly found that the disposition of the fluorescent… CONTINUE READING
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