A discrete domain of the human TrkB receptor defines the binding sites for BDNF and NT-4.

@article{Naylor2002ADD,
  title={A discrete domain of the human TrkB receptor defines the binding sites for BDNF and NT-4.},
  author={Ruth L Naylor and Alan G.S. Robertson and Shelley J. Allen and Richard B. Sessions and A. R. Clarke and Grant G. F. Mason and Judy J Burston and Sue J. Tyler and G. K. Wilcock and David Dawbarn},
  journal={Biochemical and biophysical research communications},
  year={2002},
  volume={291 3},
  pages={501-7}
}
TrkB is a member of the Trk family of tyrosine kinase receptors. In vivo, the extracellular region of TrkB is known to bind, with high affinity, the neurotrophin protein brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4). We describe the expression and purification of the second Ig-like domain of human TrkB (TrkBIg(2)) and show, using surface plasmon resonance, that this domain is sufficient to bind BDNF and NT-4 with subnanomolar affinity. BDNF and NT-4 may have therapeutic… CONTINUE READING