A direct estimation of the context effect on the efficiency of termination.

@article{Pavlov1998ADE,
  title={A direct estimation of the context effect on the efficiency of termination.},
  author={Michael Yu. Pavlov and David V. Freistroffer and Vildan Dincbas and James MacDougall and R. H. Buckingham and M{\aa}ns Ehrenberg},
  journal={Journal of molecular biology},
  year={1998},
  volume={284 3},
  pages={
          579-90
        }
}
An in vitro assay in which terminating Escherichia coli ribosomes with different stop signals in the A-site compete for a limited amount of a release factor (RF1 or RF2) has been used to estimate the relative termination efficiencies at stop codons with different adjacent downstream nucleotides. The assay allows direct measurements of relative kcat/Km parameters for the productive association of release factors to ribosomes. The kcat/Km parameter is larger for UAA(U) than for UAA(C) programmed… CONTINUE READING

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