A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis

Abstract

A simple, two-step efficient method to perform multiple-site mutagenesis of a gene from bacterial genome was developed. The method was named polyacrylamide gel electrophoresis (PAGE)-mediated overlap extension polymerase chain reaction (PCR) (POEP). The first step involves synthesis of individual fragments containing mutant sites with 15- to 25-bp overlap… (More)
DOI: 10.1007/s00253-006-0583-3

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Cite this paper

@article{Peng2006ADA, title={A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis}, author={Ri-he Peng and Ai-Sheng Xiong and Quan-hong Yao}, journal={Applied Microbiology and Biotechnology}, year={2006}, volume={73}, pages={234-240} }