A direct StyI polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test for the myophosphorylase mutation in cattle.

@article{Soethout2002ADS,
  title={A direct StyI polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test for the myophosphorylase mutation in cattle.},
  author={Ernst C. Soethout and Edward L C Verkaar and Gerhard H Jansen and Kerstin Elisabeth M{\"u}ller and Johannes A Lenstra},
  journal={Journal of veterinary medicine. A, Physiology, pathology, clinical medicine},
  year={2002},
  volume={49 6},
  pages={289-90}
}
Myophosphorylase deficiency in cattle is a muscle disease induced by a C-->T point mutation in codon 489 of the myophosphorylase gene, which until now has only been diagnosed in the Charolais breed. The disease seems to be inherited in an autosomal monogenic recessive manner. A calf of double muscled phenotype was suspected of suffering from myophosphorylase deficiency based on typical symptoms, i.e. brown-coloured, transparent urine, occurring after exercise; exercise intolerance; symptoms of… CONTINUE READING