A dipicolinic acid tag for rigid lanthanide tagging of proteins and paramagnetic NMR spectroscopy.

@article{Su2008ADA,
  title={A dipicolinic acid tag for rigid lanthanide tagging of proteins and paramagnetic NMR spectroscopy.},
  author={Xun-Cheng Su and Bradley Man and Sophie R Beeren and Haobo Liang and Shane M Simonsen and Christophe Schmitz and Thomas Huber and Barbara A Messerle and Gottfried Otting},
  journal={Journal of the American Chemical Society},
  year={2008},
  volume={130 32},
  pages={10486-7}
}
A new lanthanide tag was designed for site-specific labeling of proteins with paramagnetic lanthanide ions. The tag, 4-mercaptomethyl-dipicolinic acid, binds lanthanide ions with nanomolar affinity, is readily attached to proteins via a disulfide bond, and avoids the problems of diastereomer formation associated with most of the conventional lanthanide tags. The high lanthanide affinity of the tag opens the possibility to measure residual dipolar couplings in a single sample containing a… CONTINUE READING

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