A dimer of the lymphoid protein RAG1 recognizes the recombination signal sequence and the complex stably incorporates the high mobility group protein HMG2.

@article{Rodgers1999ADO,
  title={A dimer of the lymphoid protein RAG1 recognizes the recombination signal sequence and the complex stably incorporates the high mobility group protein HMG2.},
  author={Karla K. Rodgers and Isabelle Villey and Leon M Ptaszek and Elizabeth Corbett and David G Schatz and Joseph E. Coleman},
  journal={Nucleic acids research},
  year={1999},
  volume={27 14},
  pages={2938-46}
}
RAG1 and RAG2 are the two lymphoid-specific proteins required for the cleavage of DNA sequences known as the recombination signal sequences (RSSs) flanking V, D or J regions of the antigen-binding genes. Previous studies have shown that RAG1 alone is capable of binding to the RSS, whereas RAG2 only binds as a RAG1/RAG2 complex. We have expressed recombinant core RAG1 (amino acids 384-1008) in Escherichia coli and demonstrated catalytic activity when combined with RAG2. This protein was then… CONTINUE READING