A diabatic three-state representation of photoisomerization in the green fluorescent protein chromophore.

  title={A diabatic three-state representation of photoisomerization in the green fluorescent protein chromophore.},
  author={Seth Olsen and Ross H. McKenzie},
  journal={The Journal of chemical physics},
  volume={130 18},
  • S. OlsenR. McKenzie
  • Published 22 February 2009
  • Physics, Chemistry
  • The Journal of chemical physics
We give a quantum chemical description of the photoisomerization reaction of green fluorescent protein (GFP) chromophores using a representation over three diabatic states. Photoisomerization leads to nonradiative decay, and competes with fluorescence in these systems. In the protein, this pathway is suppressed, leading to fluorescence. Understanding the electronic states relevant to photoisomerization is a prerequisite to understanding how the protein suppresses it, and preserves the emitting… 

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