A device for the simple and rapid transcervical transfer of mouse embryos eliminates the need for surgery and potential post-operative complications.

@article{Green2009ADF,
  title={A device for the simple and rapid transcervical transfer of mouse embryos eliminates the need for surgery and potential post-operative complications.},
  author={Michael J Green and Shannon Bass and B. Spear},
  journal={BioTechniques},
  year={2009},
  volume={47 5},
  pages={919-24}
}
We describe a novel device that can be used for the transcervical transfer of embryos into pseudopregnant female mice. This nonsurgical embryo transfer (NSET) device is as efficient as standard surgical embryo transfer in the production of transgenic mice, and can also be used for the transfer of embryonic stem cell-containing chimeric blastocysts and cryopreserved embryos. In addition to the elimination of surgery, recipient females do not have to be anesthetized. The NSET device eliminates a… CONTINUE READING