A detailed analysis of cyclin A accumulation at the G(1)/S border in normal and transformed cells.

@article{Erlandsson2000ADA,
  title={A detailed analysis of cyclin A accumulation at the G(1)/S border in normal and transformed cells.},
  author={Fredrik Erlandsson and Clas Linnman and Sven Ekholm and Eva Bengtsson and Anders Zetterberg},
  journal={Experimental cell research},
  year={2000},
  volume={259 1},
  pages={86-95}
}
The temporal relationship between cyclin A accumulation and the onset of DNA replication was analyzed in detail. Five untransformed and nine transformed asynchronously growing cell cultures were investigated using a triple immunofluorescence staining protocol combined with computerized evaluation of staining intensities in individual cells. The simultaneous staining of BrdU, cyclin A, and cyclin E made it possible to determine the cell cycle position of each cell investigated. Cells at the G(1… CONTINUE READING