A degenerate primer allows amplification of part of the 3'-terminus of three distinct carlavirus species.

@article{Gaspar2008ADP,
  title={A degenerate primer allows amplification of part of the 3'-terminus of three distinct carlavirus species.},
  author={Jos{\'e} O. Gaspar and Priscila Belintani and Alvaro M R Almeida and Elliott Watanabe Kitajima},
  journal={Journal of virological methods},
  year={2008},
  volume={148 1-2},
  pages={283-5}
}
Sequences of the coat protein amino acids of definitive and tentative species of carlaviruses deposited in GenBank were aligned and a region of seven amino acids (GLGVPTE) was found to be conserved. The corresponding nucleotides were aligned, allowing the design of a degenerate primer that together with an oligo dT anti-sense primer, was effective for the detection of three distinct carlavirus species, two transmitted by aphids and one by whitefly. These primers have the advantage that about… CONTINUE READING
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