A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells.

@article{Lu2014ADX,
  title={A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells.},
  author={Hong Fang Lu and Chou Chai and Tze Chiun Lim and Meng Fatt Leong and Jia Kai Lim and Shujun Gao and Kah Leong Lim and Andrew C A Wan},
  journal={Biomaterials},
  year={2014},
  volume={35 9},
  pages={2816-26}
}
A defined xeno-free system for patient-specific iPSC derivation and differentiation is required for translation to clinical applications. However, standard somatic cell reprogramming protocols rely on using MEFs and xenogeneic medium, imposing a significant obstacle to clinical translation. Here, we describe a well-defined culture system based on xeno-free media and LN521 substrate which supported i) efficient reprogramming of normal or diseased skin fibroblasts from human of different ages… CONTINUE READING
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